Willie McCreery - the leading Irish flat trainer who is a master at the game of patience
By Lissa Oliver
Master at the game of patience
There is nothing superficial about Rathbride Stables, a traditional yard on the very coalface of the history-steeped Curragh Plains of Kildare. And there’s nothing superficial about Willie McCreery, either. Like most modern yards, the atmosphere is relaxed. McCreery appears laid back, and the staff arrive at 7 a.m. and calmly set about their tasks—the routine as smooth as a well-oiled machine. There are no instructions being given and none needed; teamwork is at its finest.
But there’s a keenness here, too—a simmering energy beneath the calm exterior. McCreery is quietly watchful and aware of every nuance. This is a man who loves what he does and just happens to be very good at it, too. The Wall of Fame in the office bears testament to the success—no better example being Fiesolana, the Gp1 Matron Stakes heroine. Improving every season and gaining a first Gp1 win for McCreery and his team as a five-year-old, she’s a good example, too, of McCreery’s patience and expertise with fillies and older horses, for which he has gained something of a reputation. Her now five-year-old Galileo colt, Up Helly Aa, is also keeping Group company under the tutelage of McCreery.
This is thanks largely to the patronage of owner-breeders. While some trainers survive on sharp two-year-olds and trading, McCreery acknowledges that having the perceived luxury of time with horses that have a longer career ahead comes with its own challenges.
‘Not selling horses and training for owner-breeders brings even more pressure’, he points out. ‘A win becomes more important, and then when they’ve had their win, you’re looking for black type. If they don’t get a win, it’s worse than if they don’t race at all; poor performances run the risk of devaluing the whole family. It comes down to making the right call, being sure enough from what they’re doing at home that they can do well and improve the family; or maybe having to risk the decision not to run them’.
Rathbride Stables, once home to Flashing Steel and where the Irish Grand National winner is now buried, has been home to McCreery since 2010. He had taken out a licence and sent out his first winner two years earlier. The original loose boxes are companion boxes, with the window through to the next box at the feed trough. ‘They can have a chat with their neighbour and have a nibble at the same time’, McCreery says. ‘It encourages them to feed, and I’m a fan of anything that gets them eating well.
‘I feed as best as I can. I hope to be second to none in that respect. I use a combination of Connolly’s Red Mills mix and nuts, and also alfalfa imported from Italy. I’ve picked it up along the way. I’m a firm believer in staying ahead of your feed, and if a horse is a little off, cut back straight away. I feed four times a day’.
McCreery starts his day in the yard at 6 a.m. with the first of the feeds, and he’ll turn out any of the horses not working that morning. That day’s runners will go out in the paddocks in the evening after returning from the races and again the next morning. Rathbride has 40 acres of turnout paddocks, as well as a 400m covered wood chip ride, and is currently home to 60 horses.
It’s ideally situated and well chosen. ‘We can just walk across to the gallops; we’ve the vet just beside us, and we’re within an hour of six tracks. As an example, I had a horse injured on the Old Vic gallop at 7.40 a.m. and he was X-rayed, diagnosed and back in his box—all by 9 a.m. I’m very lucky; any issue at all, and I have the vet with me in five minutes. I can drop blood samples in and have the results in 10 minutes’.
In the pre-COVID days, McCreery liked to give his horses days out, particularly at Dundalk where they could walk round the parade ring in front of a race crowd and gain experience. He also found the local equine pool a great help for horses who enjoyed it, but that’s now closed. ‘I would love to be near a beach; sea water does a great job’, he says. ‘But I’m not a fan of spas. They can mask the injury, and the problem is still going to be there. They’re okay for sore shins’.
Training racehorses was always in his blood; McCreery’s father Peter had enjoyed great success as a National Hunt trainer, and his older brother Peter Jr by coincidence sent out Son Of War to win the Irish Grand National the year before Flashing Steel’s success. We might wonder why McCreery chose the Flat in preference….
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How breastplates and breast girths can inhibit performance
By Dr Russell Mackechnie-Guire
Using pressure mapping and gait analysis technology, scientists have now measured how breastplates and breast-girths can compromise the horse’s jump and stride at gallop.
Breast girths and breastplates are routinely used to prevent the saddle moving backwards at home on the gallops, and on the track. Post-race bruising to the pectoral muscles, sternal abrasions, soreness and even lacerations caused by a breastplate are often accepted consequences of keeping the saddle in place. But evidence is mounting that, in addition to the physical factors, a poorly designed breastplate or badly fitted breast-girth could also be restricting the horse’s gallop and compromising its jump during a race, therefore impacting the horse’s locomotor efficiency, performance and athletic potential.
Recent research in the sports horse has demonstrated that breastplates have a significant negative effect on the horse’s action over a fence. A further pilot study looking at how breast girths and breastplates affect the racehorse indicates that they influence the movement of the shoulder and forelimbs whilst galloping, potentially comprising gallop efficiency.
Jumping short
PIC 1– Pliance pressure mapping revealed the point of peak pressure common to all breastplates in the test occurred at the point of take-off.
In the sports horse jumping study, scientists used Pliance pressure mapping (pic 1) to identify areas of peak pressure beneath traditionally fitted breastplates. A sensor mat placed between the breastplate and the horse’s skin recorded levels of pressure throughout the jump cycle. Regardless of breastplate design, the highest pressures were consistently seen in the centre of the chest (located on the midline over the sternal region), at the moment of take-off as the horse’s shoulder, elbow and knee were flexed and the shoulder was in its most forward position (point P, pic 2).
PIC 2 - Without a breastplate (top), the horse’s jump forms a smooth parabolic trajectory over the fence from take-off to landing (A).
Alongside pressure mapping, two-dimensional gait analysis was utilised to determine how pressures created by breastplates affected jumping technique. Markers were placed on the horse’s joints, and the horse’s jump was analysed at a rate of 300 frames a second—approximately 25 times faster than the human eye. The data quantified any changes in joint and limb angles. The findings demonstrated that the whole jump is adversely affected by the breastplate design and resultant pressures from the point of take-off to, and including, the stride immediately after landing.
Without a breastplate, the horse’s natural jump is a parabolic (symmetrical) curve, with the highest point an equal distance between take-off and landing. In the study, a breastplate was shown to shorten the horse’s landing position by 0.5m compared to landing with no breastplate.
With a breastplate (bottom), the horse’s lead foot makes contact with the ground much closer to the fence (B), and the landing phase of the jump is steeper.]
This pattern of an altered trajectory was scientifically recorded under experimental conditions over one single oxer fence (1.2m) on a level surface. Applying the same principles to the racehorse, the negative impact that this would have when galloping would be magnified. If the horse’s trajectory over a brush fence is shortened by the same distance as it is over a 1.2m jump, this is likely to have a significant effect on gallop efficiency—in particular stride rate, length and frequency. Over a course of 12 National Hunt fences, if half a metre of ground is lost (as a function of the altered landing trajectory) at every jump, this would require six metres to be made up over ground—potentially the length of a winning stride.
Crash landing….
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Doping control in European racing the role played by the EHSLC
By Dr Paull Khan
AN Q&A INTERVIEW WITH HENRI POURET,
CHAIR OF THE EUROPEAN HORSERACING SCIENTIFIC LIAISON COMMITTEE
In this issue, we continue our series of Question and Answer sessions with the chairs of international committees in the European and Mediterranean regions. We began (Issue 72) with the subject of classifying the major, Black Type races across Europe, with our interview with the chair of the European Pattern Committee, Brian Kavanagh. Here, we move on to the subject of doping control, which is the remit of the European Horserace Scientific Liaison Committee. Its chair, Henri Pouret, answers your questions.
The EHSLC lists these amongst its Terms of Reference:
With the aim of achieving uniformity of approach, to provide advice to the Racing Authorities of the member countries on policy, scientific and procedural matters concerning the Rules of Racing as they relate to prohibited substances.
To recommend alterations to the Rules of Racing as they relate to prohibited substances.
To recommend common policies and procedures where appropriate in the areas of sample collection, sample testing (including confirmatory analysis) and prohibited substances, and to monitor compliance by the member countries with these policies and procedures.
To agree whether specified drugs fall within the List of Prohibited Substances.
To recommend the need, where appropriate, for new or varied threshold levels, for inclusion in the Rules of Racing.
To promote liaison and discussion between the official racing laboratories and the official racing veterinary surgeons of the member countries.
To promote inter-laboratory drug testing programmes, and to monitor the results vis-à-vis the official racing laboratories of the member countries.
To agree with research priorities and to promote joint approaches, where appropriate, for their achievement.
To publish detection periods, agreed jointly between the official racing laboratories and other interested parties in the member countries for therapeutic drugs commonly used in the horse.
To exchange drug intelligence and other relevant information between the member countries.
Pouret, who has a background in law, is the Deputy CEO of France Galop, in charge of racing and also represents France on the International Federation of Horseracing Authority’s (IFHA’s) Harmonisation of Racing Rules Committee.
Q: Let’s get some definitions out of the way first. One of the EHSLC’s Terms of Reference is ‘to recommend the need, where appropriate, for new or varied threshold levels, for inclusion in the Rules of Racing’. What is the difference between a ‘threshold level’ and a ‘screening limit’?
‘Threshold level’ and ‘screening limits’ are two critical indicators for doping control determined in urine and/or plasma.
A ‘threshold level’ is a numerical figure adopted by racing authorities for endogenous substances produced by horses and for some plants traditionally grazed and harvested to horses as feed. International thresholds are recommended by the IFHA’s Advisory Council on Equine Prohibited Substances and Practices and approved by the IFHA Executive Council.
A ‘screening limit’ (SL) is also a numerical figure determined by experts for legitimate therapeutic substances. Some are harmonised internationally and some are harmonised regionally (e.g,. within EHSLC for Europe).
Both ‘threshold level’ and ‘screening limit’ are applied by racing laboratories as a reference for the reporting of positive findings.
Q: On the IFHA website, there are lists published of ‘international screening limits’ and ‘residue limits’. How do these differ?…
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Prick test: Could the ancient Chinese therapy of acupuncture be a trainer’s secret weapon?
By Alysen Miller
At first glance, the Curragh (Ire) based trainer Michael Grassick Jr. may appear to have little in common with NBA legend Shaquille O’Neal. Yet both have embraced a practice derived from traditional Chinese medicine in their quests to leave no margin left ungained when it comes to minimising pain and maximising performance.
Acupuncture may be a controversial subject for some within the equestrian community, but its potential to treat illness and injury and alleviate pain in horses is increasingly being recognised.
“My father used to use it a lot when he was training, so when I took over [in 2013], I continued it,” says Grassick Jr. “I found it very successful. If the lads feel something isn’t quite right, like they’re leaning a little bit or hanging a little bit, then you call the physio. He will pinpoint the area, and we’ll work on that area and usually you wouldn’t need him to look at it again.”
“It’s something I was interested to witness—seeing them, how they respond,” he continues. “You’d see they’d be a lot freer in themselves.”
Although acupuncture has been part of the programme for the equine inhabitants of his family Fenpark Stables for a number of years, it was a brush with Bell’s Palsy that finally convinced Grassick of the benefits of the technique. “One side of my face went numb on me about five or six years ago. They put me on drugs, but the only thing that really got it back 100% was acupuncture.”

So what exactly is acupuncture, and how does it work? Here comes the science bit—concentrate. Acupuncture works by stimulating the sensory nerves under the skin and muscles. Tiny intradermal needles penetrate the skin just enough to stimulate collagen and elastin production—two of the main structural proteins in the extracellular matrix. During this process, the acupuncturist may feel the needle being gripped by the surrounding tissue —a phenomenon known as ‘needle grasp’. A 2001 study by the University of Vermont College of Medicine further revealed that gently manipulating the needles back and forth causes connective tissues to wind around the needle—think spaghetti twirling around a fork—and sends a signal to the fibroblasts (a type of cell that produces the structural framework for such tissues) to spread and flatten, promoting wound healing.
But wait, there’s more. Under MRI, it has been shown that acupuncture causes the body to produce pain-relieving endorphins. Furthermore, it is believed that acupuncture stimulates the central nervous system. This, in turn, releases chemicals into the muscles, spinal cord and brain. These biochemical changes may further help the body’s healing process.
So what’s not to like? According to the British-based acupuncturist, Dietrich Graf von Schweinitz, the scientific benefits of acupuncture have been lost in translation. ‘The trouble with acupuncture is that it has a messy historical baggage’, explains Graf von Schweinitz, ‘that led the Western world to believe that this was metaphysical, spiritual, “barefoot doctor’ territory”’. ‘Qi’ (pronounced “chee”) may be best known as the last refuge of a scoundrel in Scrabble, but in traditional Chinese medicine, the concept of qi refers to the vital life force of any living being. Traditional Chinese medicine practitioners believe the human body has more than 2,000 acupuncture points connected by pathways, or meridians. These pathways create an energy flow—qi—through the body which is responsible for overall health. Disruption of this energy flow can, they believe, cause disease. Applying acupuncture to certain points is thought to improve the flow of qi, thereby improving health. Although in this sense, qi is a pseudoscientific, unverified concept; this linguistic quirk has meant that medical science has been slow to embrace the very real physiological benefits of acupuncture. ‘The ability of neuroscience to unravel more and more of acupuncture physiology is becoming quite staggering’, says Graf von Schweinitz.
A softly spoken American, full of German genes whose accent betrays only the slightest hint of a southern drawl, Graf von Schweinitz was an equine vet for 30 years until he sold his practice to focus on animal acupuncture. ‘I grew up on a farm in Georgia. My parents both came from rural farming backgrounds. So I was around horses all my life. I actually had my first taste of acupuncture at vet school. In my final year there was an acupuncture study going on in the clinics on horses with chronic laminitis or chronic navicular’. Like Grassick, he has personally experienced the benefits of the technique. ‘In my first job as a vet, I got kicked and was treated by a client who was an acupressurist [a close cousin of acupuncture that involves pressing the fingers into key points around the body to stimulate pain relief and muscle relaxation]. The result in terms of pain control was so bizarre and staggering I just thought, “I’ve got to know more about this”, and started my mission’.
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What does the future hold for Maisons-Laffitte racecourse and training centre?
By Katherine Ford
A visitor arriving at Maisons-Laffitte from Paris is greeted as he crosses the bridge over the Seine by a spectacular view of the town’s 15th century baroque chateau. Signage announces arrival in ‘Maisons-Laffitte, Cité du Cheval’.
The racecourse is currently inactive, but the town still revolves very much around the horse, of which approximately 1,000—half of them racehorses—are stabled in the wooded parkland. Supporters of the racecourse hope that a project will be validated to see the track reopen as soon as 2023, while the training community is revitalised following a 1.5 million Euro investment in facilities, and the team is keen to attract new professionals.
France Galop CEO Oivier Delloye explains, ‘The situations with the racecourse and the training centre are two separate subjects. France Galop is focused upon ensuring continuity for the training centre. We have not closed the door to the possibility of racing returning to Maisons-Laffitte, but if this does happen, it will not be organised by France Galop.
‘There is no racing currently planned for Maisons-Laffitte, and none for next year or in the future unless new elements enter the picture. France Galop is working in partnership with the town council because if there is to be a future for the track, it will certainly involve both parties; and the mayor is very keen for the racecourse to re-open. The idea is to seek a new economic model to make use of the buildings and facilities and therefore finance their upkeep, while France Galop would contribute the prize money and a share of the maintenance costs. This is the model used for provincial racecourses in France, and the system works; however, the costs involved in Maisons-Laffitte are higher than at the country tracks. It is important that any racing at Maisons-Laffitte in the future be organised to a high standard in accordance with the track’s status as a Parisian and premium track.
‘Earlier this year, France Galop published a call for expressions of interest and will analyse which projects could be compatible with a commercial exploitation of the racecourse and buildings, in conjunction with the organization of racing. We have received a number of dossiers including some quite creative concepts—from varying profiles of operators to envisaging different ways of promoting the site, which is recognised as being exceptional. The responses are a mixed bag with some quite structured and some more exotic ideas. We hope to be able to select one or two projects to work on a viable business plan. France Galop will not validate any project unless we are certain of its financial sustainability as the last thing we want is for the racecourse to open and then close again shortly after, or for the new operator to lose money and call in the racing authorities to help out. The whole process will be carried out in full consultation with the town of Maisons-Laffitte and in a best-case scenario, racing could return in 2023’.
Ironically, when the racecourse was closed by France Galop to reduce expenditure, the French racing authority announced an ambitious investment programme of 1.5€ million to modernise and improve the town’s racehorse training facilities. Olivier Delloye is pleased with the results: ‘I visited Maisons-Laffitte last week and found that there was a very positive atmosphere with trainers optimistic about the future. It is clear that the professionals based in Maisons-Laffitte have not lost clients due to the closure of the racecourse, and recent results show that the facilities are perfectly adapted for training all types of horses—from juveniles to top milers and the best steeple-chasers of Auteuil’.
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Nutrition Analysis - Understanding equine feed labelling
By Dr Catherine Dunnett, BSc, PhD, R.Nutr
Understanding a bit about feed labelling and feed manufacturing is worth the drudge, as it can help you make better choices for your horses in training and maybe even save a few pounds or dollars. Whilst the information that a feed manufacturer must legally provide can vary from country to country, it is broadly similar. The purpose of feed labelling is primarily to give information about the feed to a potential customer, allowing informed choices to be made. However, it also provides a measure against which legislators and their gatekeepers can ensure feed manufacturing is consistent and that the feed is not being misrepresented or miss-sold.
The on-bag information is most often separated into what’s known as the statutory statement (or the legally required information) and then other useful information which features outside of the statutory statement. The statutory information can be found in a discrete section of the printed bag, or it could be located on a separate ticket, stitched into the bag closure. Whichever is the case, this is the information legally required by the country’s legislators and which the feed manufacturer is legally bound to adhere to. Typically, the information required within the statutory statement includes for example:
Name, address and contact details of the company responsible for marketing and sale of the feed.
The purpose of the feed, for example for pre-training or racing.
Reference to where the feed has been manufactured. Some companies do not have their own manufacturing facility and will use a contract manufacturer. In the UK, a feed mill manufacturing feed must be registered and on the UK list of approved feed business establishments and there is a number, colloquially known as a GB number, which refers to the feed mill’s registration. A useful snippet is that if this GB number changes on pack, this may mean that the manufacturer has switched to a different mill.
A list of ingredients in the feed in order of inclusion. The first ingredient will have the highest level of inclusion and the last being the least level.
A declaration of analysis, which is used to describe the nutritional characteristics of the feed is quite limited in what can legally be declared. There is a predefined legally binding list of analytes that must be declared in this section, which depends on the type of feed. For example, this might include percentage protein, oil, crude fibre, ash, as well as the level of added additives such as copper, vitamins A, D and E, as well as any live microbiological ingredients, or preservatives, binders etc. In addition, the analysis must be carried out using specific laboratory methodologies set out in the legislation. Feed manufacturers are allowed some tolerance on analysis, or limits of variation around their declaration to account for variation in sampling and manufacturing as well as the analytical variation itself and this can be as high as 10-20% in some instances for example.
The level and source of additives. For example, added copper must be declared and the level (mg/kg) and source (copper sulphate or if as a chelate, copper chelate of amino acid hydrate) stated.
Any additives (i.e., ingredients that don’t contribute to the nutritional value of the feed) can only be used if they appear on an authorised list of additives—meaning they have passed scrutiny for safety and efficacy. This list of additives pre-Brexit was maintained by the EU and since Brexit, whilst we can theoretically modify on our own terms, the reality is that we have largely adopted the EU list.
There is a lot of useful information that is not legally allowed within the statutory statement that you will often find on a separate section of the bag, or indeed on a company website. For example, other analyses such as percentage of starch and sugar are often useful when choosing an appropriate feed and an estimate of the level of digestible energy (DE MJ/kg) is also helpful. Feeding guides also generally appear outside the statutory statement and can be quite useful. Whilst I am a firm believer in looking at the horse to help set the required amount of feed, feeding guides do give vital information, particularly about the likely minimum amount of this feed required to deliver a suitable level of vitamins and minerals.
When being first really counts…
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TopSpec Trainer of the Quarter - Richard Phillips
Britain's first ever National Racehorse Week will take place 12-19 September this year, thanks to the brainchild of Moreton-in-Marsh, Gloucestershire trainer, Richard Phillips. When fan pressure led to singer Taylor Swift withdrawing from performing at the Melbourne Cup, Phillips became aware how little people know about racing. ‘If they knew more about it, they would be more comfortable about racing’, he says.
Phillips has been training since 1993, enjoying success with star chasers Time Won’t Wait, Gnome’s Tycoon and Noble Lord, while La Landiere provided his first Cheltenham Festival winner. He has also won awards for his charity work, including the 2021 Community Award at the Godolphin Stud and Stable Staff Awards, the 2014 Voluntary Award at the Animal Health Trust Awards and the 2011 Pride Of Racing Award from Racing Welfare.
‘I am delighted to see the idea of National Racehorse Week spring into life. It is a fantastic opportunity for our owners, staff, trainers and jockeys to come together for a common love of the racehorse. Racing can sometimes be divided, but the one thing we all agree on is our love of the horse. National Racehorse Week will be our chance to show the public and policy makers that racing has absolutely nothing to hide. We have a great story to tell, so let's get it out there and tell it’.
National Racehorse Week has gathered momentum and support from across the racing industry and is funded by the Racing Foundation, The Sir Peter O'Sullevan Charitable Trust and Great British Racing, in partnership with the National Trainers Federation. It is also the first public engagement of the Horse Welfare Board's Equine Welfare Communications Strategy, funded by the HBLB. Phillips is particularly keen to see MPs involved and visiting their local yards. ‘If racing is then discussed, they will be in a position to know more about it’.
As he points out, you never know who might walk into your yard and the influence that may have on them in the future. ‘The public sometimes thinks racing and racehorse ownership is not accessible, but it is and this is our opportunity to show that. So many of us do so much every day and put the health of our horses before our own. This is our chance to engage with local people, invite them into our yard to meet our staff and get into conversation with them’.
Rupert Arnold, Chief Executive of the National Trainers Federation, is equally enthusiastic. ‘There has been overwhelming and enthusiastic support from trainers. Everyone wants a chance to show their respect for the racehorses to whom they give such exceptional care. I am confident that trainers and their staff, who forge such a close bond with their horses, will grasp the opportunity provided by National Racehorse Week’.
It's not too great a leap of imagination to see National Racehorse Week becoming International Racehorse Week, or at the very least European Racehorse Week in years to come. It is a concept easily adopted by other countries and could work in tandem with existing stallion trail weekends, incorporating every aspect of the thoroughbred’s life— ‘from cradle to grave, a life well lived’, as Phillips says.
‘It’s everybody’s duty to do something and it should be enjoyable, not a chore. We can show people how much we do for horses and how much they do for us. Celebrating the racehorse benefits everyone’.
If you are a trainer and interested in finding out more information please contact Harriet Rochester, harriet@nationalracehorseweek.com
To register visit www.nationalracehorseweek.uk
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Can we use biomarkers to predict catastrophic racing injuries in thoroughbreds?
Promising developments in quest to prevent catastrophic racehorse injuries
University of Kentucky study shows association between mRNA biomarkers and catastrophic injuries in Thoroughbred racehorses—a positive step forward in the development of a pre-race screening tool.
By Holly Weimers
Catastrophic injuries in Thoroughbred racehorses is a top-of-mind concern for the global racing industry and its fans. That sentiment is shared by researchers at the University of Kentucky and their collaborators, who are working to learn more about changes happening at a cellular level that might indicate an injury is lurking before it becomes career or life ending.
Could it be possible to identify an early marker or signal in horses at risk of catastrophic injury, allowing for intervention before those injuries happen? And, if so, might this type of detection system be one that could be implemented cost effectively on a large scale?
According to Allen Page, DVM, PhD, staff scientist and veterinarian at UK’s Gluck Equine Research Center, the short answer to both questions is that it looks promising.
To date, attempts to identify useful biomarkers for early injury detection have been largely unsuccessful. However, the use of a different biomarker technology, which quantifies messenger RNA (mRNA), was able to identify 76% of those horses at risk for a catastrophic injury.
An abstract of this research was recently presented at the American Association of Equine Practitioners’ annual meeting in December 2020 and the full study published January 12 in the Equine Veterinary Journal (https://beva.onlinelibrary.wiley.com/journal/20423306). In this initial research—which looked at 21 different mRNA markers selected for their roles in encoding proteins associated with inflammation, bone repair and remodeling, tissue repair and general response to injury—three markers showed a large difference in mRNA levels between injured and non-injured horses.
For almost four years, Page and his University of Kentucky colleagues have been analyzing blood samples from almost 700 Thoroughbred racehorses. The samples, collected by participating racing jurisdictions from across the United States, have come from both catastrophically injured and non-injured horses in a quest to better understand changes that might be happening at the mRNA level and if there are any red flags which consistently differentiate horses that suffer a catastrophic injury.
According to Page, the ultimate hope is to develop a screening tool that can be used pre-race to identify horses at increased risk for injury. The results of this study, which was entirely funded by the Kentucky Horse Racing Commission’s Equine Drug Research Council, suggest that analysis of messenger RNA expression could be an economical, effective and non-invasive way to identify individual racehorses at risk for catastrophic injury.
Joining Page in the research from UK’s Gluck Center are Emma Adam, BVetMed, PhD, DACVIM, DACVS, assistant professor, research and industry liaison, and David Horohov, PhD, chair of the Department of Veterinary Science, director of the Gluck Center and Jes E. and Clementine M. Schlaikjer Endowed Chair.
Previous research has shown that many catastrophic injuries occur in limbs with underlying and pre-existing damage, leading to the theory that these injuries occur when damage accumulation exceeds the healing capacity of the affected bones over time. Since many of these injuries have underlying damage, it is likely that there are molecular markers of this that can be detected prior to an injury.
The identification of protein biomarkers for these types of injuries has been explored in previous research, albeit with limited success. The focus of this project, measuring messenger RNA, had not yet been explored, however. The overall objective was to determine if horses that had experienced a catastrophic injury during racing would show increased inflammatory mRNA expression at the time of their injury when compared to similar horses who were not injured.
The genetic acronyms: A primer on DNA, RNA, mRNA and PCR
This research leverages advances made in genetics during the last several decades, both in a greater understanding of the field as well as in applying that knowledge to specific issues facing the equine industry, including catastrophic breakdown in racehorses.
The genetic code of life is made up of genes and regulatory elements encoded by DNA, or deoxyribonucleic acid, which is found in the nucleus of cells in all living organisms. It is arranged in a double helix structure, similar to a twisted ladder. The rungs of that ladder are nucleotide base pairs, and the ordering of those base pairs results in the specific genetic code called a gene. The genetic code in the genes and the DNA tell the body how to make proteins.
RNA (ribonucleic acid) is created by RNA polymerases, which read a section of DNA and convert it into a single strand of RNA in a process called transcription. While all types of RNA are involved in building proteins, mRNA is the one that actually acts as the messenger because it is the one with the instructions for the protein, which is created via a process called translation. In translation, mRNA bonds with a ribosome, which will read the mRNA’s sequence. The ribosome then uses the mRNA sequence as a blueprint in determining which amino acids are needed and in what order. Amino acids function as the building blocks of protein (initially referred to as a polypeptide). Messenger RNA sequences are read as a triplet code where three nucleotides dictate a specific amino acid. After the entire polypeptide chain has been created and released by the ribosome, it will undergo folding based on interactions between the amino acids and become a fully functioning protein.
While looking at inflammation often involves measuring proteins, Page and his collaborators opted to focus on mRNA due to the limited availability of reagents available to measure horse proteins and concerns about how limited the scope of that research focus would be. Focusing on mRNA expression, however, is not without issues.
According to Page, mRNA can be extremely difficult to work with. “A normal blood sample from a horse requires a collection tube that every veterinarian has with them. Unfortunately, we cannot use those tubes because mRNA is rapidly broken down once cells in tubes begin to die. Luckily, there are commercially-available blood tubes that are designed solely for the collection of mRNA,” he said.
“One of the early concerns people had about this project when we talked with them was whether we were going to try to link catastrophic injuries to the presence or absence of certain genes and familial lines. Not only was that not a goal of the study, [but] the samples we obtain make that impossible,” Page said. “Likewise for testing study samples for performance enhancing drugs. The tubes do an excellent job of stabilizing mRNA at the expense of everything else in the blood sample.”
In order to examine mRNA levels, the project relied heavily on the ability to amplify protein-encoding genes using a technique called the Polymerase Chain Reaction (PCR). By using a variety of techniques, samples from the project were first converted back to DNA, which is significantly more stable than mRNA, and then quantified using a specialized machine that is able to determine the relative amount of mRNA initially present in the individual samples. While it is easy to take for granted the abilities of PCR, this Nobel Prize winning discovery has forever changed the face of science and has enabled countless advances in diagnostic testing, including those used in this study.
The research into mRNA biomarkers….
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